STEM / applied
Biochemistry I · Undergraduate · Chemistry
Topics
Laboratory and analytical biochemistry
- Protein purification: chromatography, electrophoresis, centrifugation
- SDS-PAGE and Western blotting (introduction)
- Spectrophotometric protein assays: Bradford, BCA
- Enzyme activity assays and kinetic data analysis
- Buffer preparation for biochemical experiments
- pH and ionic strength effects on enzyme activity
- Dialysis, ultrafiltration, and concentration methods
- Safe handling of biological samples and biohazards
- Recombinant protein expression overview
- Data presentation for biochemical experiments
Applied biochemistry
- Clinical biochemistry: blood panels and biomarkers
- Pharmaceutical enzyme targets and drug design (intro)
- Industrial enzymes: detergents, food processing
- Metabolic diseases: diabetes, PKU, lysosomal storage disorders
- Sports biochemistry: energy systems and nutrition
- Agricultural biochemistry: nitrogen fixation, photosynthesis efficiency
- Biotechnology products: insulin, vaccines, monoclonal antibodies
- CRISPR and genetic engineering (biochemical perspective)
- Nutritional biochemistry: vitamins and minerals as cofactors
- Case studies linking structure to disease mechanism
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$60.00 · 60 min · Undergraduate · Online ($60/hr)
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