STEM / applied
Biochemistry I laboratory · Undergraduate · Chemistry
Learning objectives from the Biochemistry I laboratory syllabus, grouped by unit. Click an objective for study materials.
Learning objectives
Click an objective for study materials.
Buffers and biomolecule handling
- Prepare biological buffers — Prepare biological buffers
- Measure and adjust pH carefully — Measure and adjust pH carefully
- Handle proteins/enzymes with cold-chain awareness — Handle proteins/enzymes with cold-chain awareness
- Avoid contamination — Avoid contamination
- Record buffer recipes reproducibly — Record buffer recipes reproducibly
Protein quantification
- Bradford/BCA/Lowry-style assays as available — Bradford/BCA/Lowry-style assays as available
- Build standard curves for protein concentration — Build standard curves for protein concentration
- Blank complex matrices — Blank complex matrices
- Assess linearity limits — Assess linearity limits
- Report concentration with units and method — Report concentration with units and method
Enzyme activity labs
- Measure initial rates spectrophotometrically — Measure initial rates spectrophotometrically
- Vary substrate for Michaelis-Menten style plots — Vary substrate for Michaelis-Menten style plots
- Test pH or temperature effects — Test pH or temperature effects
- Include proper controls — Include proper controls
- Connect plots to Biochemistry I kinetics language — Connect plots to Biochemistry I kinetics language
UV-Vis of biomolecules
- Nucleic acid A260/A280 practice (intro) — Nucleic acid A260/A280 practice (intro)
- Chromophore assays — Chromophore assays
- Pathlength and dilution calculations — Pathlength and dilution calculations
- Instrument blank discipline — Instrument blank discipline
- CER write-ups linking spectra to structure — CER write-ups linking spectra to structure
What each unit includes
Open a unit below for full materials. Typical resources:
- Study guide
- Exam Strategy
- Common Mistakes
- Worksheets
- Word problems
- Mixed Practice
- Multi-Unit Problems
- Review
- Practice test
- Answer key
Study units
Each unit includes a study guide, worksheets, review, practice test, and answer key. One unit is free; subscribe for the full class.
- Buffers and biomolecule handlingComing soon
- Protein quantificationComing soon
- Enzyme activity labsComing soon
- UV-Vis of biomoleculesComing soon
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$1,162 · Biochemistry I laboratory · 18 tutoring hrs
Study guides, worksheets, reviews, practice tests, and answer keys for 1 class. 18 tutoring hours (1 hr / week · semester). Bundle discount applied vs buying separately. Pay in full via Zelle or Venmo.