Standard syllabus
Biochemistry II laboratory · Undergraduate · Chemistry
Learning objectives from the Biochemistry II laboratory syllabus, grouped by unit. Click an objective for study materials.
Learning objectives
Click an objective for study materials.
Metabolic and pathway assays
- Assay a pathway enzyme or metabolite (as scheduled) — Assay a pathway enzyme or metabolite (as scheduled)
- Use coupled assays when appropriate — Use coupled assays when appropriate
- Normalize activity to protein — Normalize activity to protein
- Discuss physiological context carefully — Discuss physiological context carefully
- Control for non-enzymatic background — Control for non-enzymatic background
Nucleic acid basics
- Pipette small volumes accurately — Pipette small volumes accurately
- Spectrophotometric DNA/RNA quant (intro) — Spectrophotometric DNA/RNA quant (intro)
- Gel electrophoresis when available — Gel electrophoresis when available
- Label and store nucleic acid samples — Label and store nucleic acid samples
- Connect to Biochemistry II information topics — Connect to Biochemistry II information topics
Biomolecule separations
- Size-exclusion or ion-exchange teaching labs — Size-exclusion or ion-exchange teaching labs
- Track fractions by assay or A280 — Track fractions by assay or A280
- Estimate purity improvements — Estimate purity improvements
- Document column parameters — Document column parameters
- Relate elution to physical properties — Relate elution to physical properties
Multi-week biochemistry project
- Plan a short inquiry with controls — Plan a short inquiry with controls
- Combine assay, separation, and report — Combine assay, separation, and report
- Present quantitative conclusions — Present quantitative conclusions
- Troubleshoot a failed trial — Troubleshoot a failed trial
- Bridge toward research lab expectations — Bridge toward research lab expectations
What each unit includes
Open a unit below for full materials. Typical resources:
- Study guide
- Exam Strategy
- Common Mistakes
- Worksheets
- Word problems
- Mixed Practice
- Multi-Unit Problems
- Review
- Practice test
- Answer key
Study units
Each unit includes a study guide, worksheets, review, practice test, and answer key. One unit is free; subscribe for the full class.
- Metabolic and pathway assaysComing soon
- Nucleic acid basicsComing soon
- Biomolecule separationsComing soon
- Multi-week biochemistry projectComing soon
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$1,162 · Biochemistry II laboratory · 18 tutoring hrs
Study guides, worksheets, reviews, practice tests, and answer keys for 1 class. 18 tutoring hours (1 hr / week · semester). Bundle discount applied vs buying separately. Pay in full via Zelle or Venmo.